Journal: International Journal of Molecular Medicine

Article Title: MOTS-c protects against placental injury via Nrf2 activation in hypoxia-induced intrauterine growth restriction mice

doi: 10.3892/ijmm.2025.5697

Figure Lengend Snippet: MOTS-c exposure attenuates oxidative stress in HUVECs. (A) Relative intensity of DCFH-DA staining. (B) Cellular SOD activity. (C) Cellular MDA content. (D) Representative images of MitoSOX staining. Scale bar, 10 μ m. (E) Representative images of JC-1 staining. Scale bar, 50 μ m. (F) Representative immunofluorescence images of Nrf2 in HUVECs. Scale bar, 5 μ m. (G) Relative nuclear to cytoplasm fluorescence ratio. Results are representative of three independent experiments. Data are expressed as the mean ± SD, n=4. *** P<0.001, # P<0.05 vs. PBS under hypoxic conditions, ## P<0.01 vs. PBS under hypoxic conditions; ### P<0.001 vs. PBS under hypoxic conditions. SOD, superoxide dismutase; MDA, malondialdehyde; DCFH-DA,2'7'-dichlorodihydro fluorescein diacetate ; Nrf2, nuclear factor erythroid 2-related factor 2.

Article Snippet: At room temperature, the samples were co-incubated with the corresponding fluorescence labeled secondary antibody (1:100; cat. no. 33106ES60; Shanghai Yeasen Biotechnology Co., Ltd.) for 60 min, and freshly prepared DAB (cat. no. #CW20695; CWBIO) staining was then performed.

Techniques: Staining, Activity Assay, Immunofluorescence, Fluorescence